[General] Will these be sterile after 30mins in the instant pot?
Normally I put my sterile pouches in with my grain or substrate and they get done at 15psi for 2hrs. I spaced and completely forgot to put them in last night and have to test all my LCs on agar today. Will 30 mins in the IP (with my agar) be long enough or is it too risky? I normally flame sterilize even after they've been steam sterilized bc I'm neurotic lol
Normally I put my sterile pouches in with my grain or substrate and they get done at 15psi for 2hrs. I spaced and completely forgot to put them in last night and have to test all my LCs on agar today. Will 30 mins in the IP (with my agar) be long enough or is it too risky? I normally flame sterilize even after they've been steam sterilized bc I'm neurotic lol